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肿瘤疫苗单元研究进展
   点击数:1532     更新时间:2010-11-04

      Hong L, Zhang J*, Min J, Lu JR, Li FF, Li H, Guo SP, Li Q*. A role for MHBst167/HBx in hepatitis B virus-induced renal tubular cells apoptosis. Nephrol Dial Transplant, 2010;25(7):2125-2133 
(*co-corresponding author)
      Background. The pathogenesis of hepatitis B virus (HBV)-associated glomerulonephritis (HBVGN) is generally believed to be immune complex deposition. However, the presence of HBV-DNA and -RNA in HBVGN renal tissues suggested a direct virally induced injury. We previously showed that nuclear factor κB (NF-κB) was activated in HBVGN renal tissues, especially in tubular cells. We therefore investigated the role of NF-κB in tubular epithelial cells with HBV infection. Methods. Nuclear translocation of NF-κB and alpha subunit of NF-κB inhibitor (IκBα) phosphorylation were assessed by immunodetection following transfection of HK-2 cells with mhbst167 and/or hbx. Electrophoretic mobility shift assays (EMSA) and dual luciferase reporter assays (DLR) were used to further examine NF-κB activation following transfection. Hochest 33258 and NF-κB/terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) double staining were used to detect apoptosis and the correlation between NF-κB activation and apoptosis. Protein kinase C (PKC) assay and ERK phosphorylation were assayed for a possible mechanism of NF-κB activation. Results. Cells transfected with mhbst167 and/or hbx increased NF-κB nuclear translocation, phosphor-IκBα, κB-DNA binding activity, κB-dependent transcription and apoptotic index compared to controls (P < 0.05). The nuclear distribution of NF-κB strongly correlated to cellular apoptosis. PKC activity and phosphor-ERK were also increased (P < 0.05) during the NF-κB activation process. However, all above parameters were diminished after pyrrolidine dithiocarbamate (PDTC)-incubation, a NF-κB inhibitor (P < 0.05). Conclusion. MHBst167/HBx-induced NF-κB activation via the PKC/ERK pathway in renal tubular cells undergoing apoptosis may be involved in virally induced pathogenesis.

      Zhang J, Cheng H, Qiao Q, Zhang JS, Wang YM, Fu X, Li Q. Malignant solitary fibrous tumor arising from the pineal region and literature review. Neuropathology, 2010;30:294-298
      We report a case of malignant solitary fibrous tumor involving the pineal region in a 49-year-old woman. The patient presented with headache, slowly progressive weakness of the right lower extremities and upgaze palsy over the past year. Histologically, the tumor was composed of moderately hypercellular proliferated spindle cells with eosinophilic collagen bands. These cells were diffusely and strongly immunoreactive with CD34, CD99, and vimentin, but were negative with epithelial membrane antigen, S-100 protein, Bcl-2, smooth muscle actin, cytokeratin and glial fibrillary antigenic protein. MIB-1 labeling indices and mitosis rates were 7.3 1 1.8% and 5 per 10 high power fields, respectively. Ultrastructural examination revealed that the neoplastic cells had features of fibroblastic differentiation. Differential diagnoses included fibrous meningioma and hemangiopericytoma. The present case provides one unique example of a rare entity to the already diverse spectrum of the pineal region neoplasms encountered in neuropathology.

 

 

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